Identification of sialyl Lewis antigen carrier proteins involved in the metastasis of lung cancer and development of diagnostic method to measure them

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670166
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Kochi University
• Principal Investigator: HONKE Koichi 高知大学, 教育研究部医療学系, 教授 (80190263)
• Co-Investigator(Kenkyū-buntansha): JIANG Songlin 高知大学, 学内共同利用施設等, 特任助教 (80598540)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 糖鎖 / シアリルルイスＸ / スルホルイスＸ / 肺腺がん / 血行性転移

Outline of Final Research Achievements

The sialyl Lewis X (sLeX) antigen is involved in the hematogenous metastasis of lung cancer cells. A human lung adenocarcinoma cell line ABC-1 has two sLeX carrier proteins with molecular masses of 190 kDa and more than 250 kDa. We have purified the sLeX carrier proteins using an immunoaffinity chromatography from the plasma membrane and the conditioned medium of ABC-1, and have identified protein X and Y by means of mass spectrometry.
When protein X was knockdowned by siRNA, the cell surface expression level of sLeX was unchanged. This finding suggested that the sLeX carrier protein on the cell surface was protein Y. The expression inhibitory experiments of protein Y are under way.

Free Research Field

糖鎖生物学