2014 Fiscal Year Final Research Report
Possible existence of lysosome-like organelle within mitochondria and its role in mitochondrial quality control
Project/Area Number |
25670169
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Pathological medical chemistry
|
Research Institution | National Cancer Center Japan |
Principal Investigator |
ARAKAWA Hirofumi 独立行政法人国立がん研究センター, 研究所, 分野長 (70313088)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Keywords | リソソーム / ミトコンドリア / タンパク質分解 / オートファジー / 活性酸素種 / 酸化ストレス / 酸化タンパク質 / 品質管理機構 |
Outline of Final Research Achievements |
In the present study, by performing electron microscopic and immune-electron microscopic analyses and protenase K protection assay, we confirmed that at least four lysosomal proteins (Cathepsin-B, Cathepsin-D, LAMP1 and LAMP2) are accumulated within mitochondria without destroying the mitochondrial structure in response to hypoxia in mouse embryonic fibroblasts and human normal mammary epithelial cells. Therefore, the Mieap-induced accumulation of lysosome-like organelles within mitochondria is a hypoxic-responsive physiological function in the cell, which is conserved at least from mouse to human.
|
Free Research Field |
腫瘍生物学
|