Systemic generation of metastatic models of lung adenocarcinoma

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670178
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Jichi Medical University
• Principal Investigator: niki toshiro 自治医科大学, 医学部, 教授 (90198424)
• Co-Investigator(Kenkyū-buntansha): YOSHIMOTO TAICHIRO 自治医科大学, 医学部, 講師 (20634166)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 肺癌 / 転移

Outline of Final Research Achievements

In this study, we established a series of mouse models of lung cancer metastasis by intracardiac inoculation of lung adenocarcinoma cell lines harboring various driver mutations, such as EGFR, KRAS, ALK, and MET. We also generated subclones that has the ability to grow in suspended condition but reattach in ordinary culture, by long-term, 3-dimensional culture under low-bind culture conditions. These subclones, designated FL clones, exhibited highly metastatic potential as compared to parental cell lines. We further performed comprehensive genomic analysis of the cell lines (parental and FL cell lines) and identified metastasis-related genes that showed altered expression in FL cells vs. parental cells, and genomic regions amplified in FL cell lines. Thus, the metastatic models created in the present study would be a promising new resource to study the molecular mechanisms of lung cancer metastasis that develop under various genetic background.

Free Research Field

人体病理