Identification of Cardiomyogenic Factor and Improved Efficiency of Cardiomyogenesis in Human Mesenchymal Stem Cells(MSCs)- Participation of new immune cells -

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670181
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Keio University (2014-2015); National Research Institute for Child Health and Development (2013)
• Principal Investigator: HIDA NAOKO 慶應義塾大学, 医学部, 共同研究員 (70360112)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Tetsuo 慶應義塾大学, 医学部, 准教授 (10209702)
• Co-Investigator(Renkei-kenkyūsha): UMEZAWA Akihiro 独立行政法人国立成育医療センター, 再生医療センター, センター長 (70213486)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ヒト間葉系細胞 / 羊膜 / 心筋分化 / 免疫寛容

Outline of Final Research Achievements

Human MSCs have been isolated from bone marrow, adipose tissues, cord blood, amniotic fluid, amniotic membrane, placentae, and umbilical cord tissues. Especially, amniotic membrane derived cells have the potential to differentiate into various tissues, and they are immunologically privileged and produced only minimal immune reactivity. In this study, we performed gene-expressing analysis of two types cells derived from human amniotic membrane, amniotic epithelial cells and mesenchymal stromal cells, and compare the expression data with that of human adipose tissue- and bone marrow derived mesenchymal stem cells. In the result, we found the some genes of amniotic epithelial cell- or mesenchymal stromal cell-specific expression, including the immune system and inflammation-related genes.

Free Research Field

幹細胞工学