2013 Fiscal Year Final Research Report
Development of a Subtraction Sequencing method for obtaining genomic information of parsites
| Project/Area Number |
25670200
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Keywords | ゲノム / 超並列シーケンシング |
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| Research Abstract |
The objective of the present study was to develop a subtraction sequencing method for obtaining genomic information of unicellular parasites. Escherichia coli and Saccharomyces cerevisiae DNAs were used as models for a host and parasite genome, respectively. The latter DNA was concentrated by subtracting the former DNA from the DNA mixture with biotinated E. coli library and streptavidin magnetic beads. The concentration was verified with quantitative PCR. Now this method is proven as feasible, but further elaboration will be required for practical use.
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