2014 Fiscal Year Final Research Report
Development of the in vitro maintenance system for undifferentiated stem cells using Toxoplasma ROP16
| Project/Area Number |
25670201
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | トキソプラズマ / 病原性因子 / 転写因子 / エフェクター機構 |
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| Outline of Final Research Achievements |
In the present study, we tested whether a Toxoplasma-derived virulence factor ROP16 is capable of sustaining the undifferentiated state of embryonic stem cells (ES cells). Furthermore, we searched novel Toxoplasma-derived factors that can potentiate the differentiation of ES cells. Then, we found that overexpression of ROP16 activeted Stat3, which is essential for the maintenance of the undifferentiaion of ES cells, more strongly than LIF. In addition, we succeeded to isolate GRA6 as a novel activator of host NFAT4.
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| Free Research Field |
寄生虫学
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