2014 Fiscal Year Final Research Report
Development of novel anti-virus reagent using DNA Origami
| Project/Area Number |
25670217
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IKEBUKURO Kazunori 東京農工大学, 大学院工学研究院, 教授 (70251494)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | DNA折り紙 / アプタマー / ウイルス |
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| Outline of Final Research Achievements |
In this study, we performed basic experiments regarding DNA origami, aptamer and DNA nano-structure for ability to use anti-viral reagents. Firstly, we synthesized superstructure of aptamers with avidin-biotin system against PRRSV (Porcine reproductive and respiratory syndrome virus). The superstructure had no suppressive effects on viral binding to cells as preliminary experiment. We synthesized improved novel nanostructure with aptamer and Gq-switch, which is network structure of G-quadruplex. We obtained the results that the superstructure is able to bind VEGF and FADGDH by some experiments. These studies strongly suggested a possibility that such a superstructure inhibits viral multiplication.
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| Free Research Field |
ウイルス学
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