2014 Fiscal Year Final Research Report
Further improvement of virus receptor identification system
| Project/Area Number |
25670222
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | ウイルス受容体 / シュードタイプ / 細胞傷害 |
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| Outline of Final Research Achievements |
I have established and improved experimental system based on expression cloning to identify virus receptors efficiently. The present project is to overcome a disadvantage of the system that the systems are inapplicable to viruses whose glycoproteins cannot work as envelope proteins of retro- or lentivirus vectors. Important in my system is use of viruses which don’t give infected cells damage.
I failed to overcome the disadvantage with VSV and JEV. However, expression of a reporter fCD2 could be done with M segment of SFTS virus being known to show no/low cell damage.
Reverse genetics for SFTS virus have been reported, therefore, a next step is to prepare recombinant SFTS virus stably expressing a reporter in a future project.
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| Free Research Field |
ウイルス学
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