Role of natural helper cells in adipose tissue inflammation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670235
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KOYASU Shigeo 独立行政法人理化学研究所, 統合生命医科学研究センター, センター長 (90153684)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: マクロファージ / 腸管 / 粘膜固有層 / 自然リンパ球

Outline of Final Research Achievements

γc-/-Rag2-/- mice deficient for all lymphocytes were resistant to high-fat diet (HFD)-induced obesity. Adoptive transfer of NH cells derived from small intestinal lamina propria (LP) but not those from mesentery restored weight gain of γc-/-Rag2-/- mice upon HFD. Co-cultivation of macrophage with NH cells in vitro increased anti-inflammatory M1 macrophages as expected. On the other hand, adoptive transfer of LP NH cells into γc-/-Rag2-/- mice resulted in the increase of pro-inflammatory M1 macrophages as well as eosinophils in adipose tissues in vivo. These results suggest that NH cells are involved in HFD-induced obesity and adipose tissue inflammation through mechanisms distinct from type 2 cytokine-mediated pathway.

Free Research Field

医歯薬学