Development of a noninvasive method for beta cell mass measurement using a nuclear magnetic resonance

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670258
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied pharmacology
• Research Institution: Kyoto University
• Principal Investigator: INAGAKI Nobuya 京都大学, 医学(系)研究科(研究院), 教授 (30241954)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Tetsuya 京都大学, 大学院医学研究科, 教授 (00209561) ; TOYODA Kentaro 京都大学, 医学部附属病院, 講師 (00447971) ; KIMURA Hiroyuki 京都薬科大学, 薬学部, 准教授 (50437240)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 膵島イメージング / 19F-MRI

Outline of Final Research Achievements

We used an exendin(9-39), which bind to GLP-1 receptor specifically, as a basic frame of probe. As a results of probe characteristic analysis, we could show a specific binding against GLP-1 receptor. However, 19F peak derived from probe could not be detected on islets cells and MIN6 cells. Therefore, we synthesized a new probe used an exendin4. We analyzed the new probe using same methods. In phantom experiment of the probe using 19F-MRS, two peaks were detected. We identified a peak derived from probe with 19F because we could show that a one of the peaks was derived from TFA. However, we could not detect a peak of probe on islets and INS-1cells due to insufficiency number of 19F molecules. Though we could not establish a MR imaging probe in this period, we plane a new probe to establish the probe.

Free Research Field

糖尿病