The development of novel internal radiation dosimetry using circulating exosomal RNAs into body fluids

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670264
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Hirosaki University
• Principal Investigator: CHIBA Mitsuru 弘前大学, 保健学研究科, 講師 (20583735)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Toshiya 弘前大学, 大学院保健学研究科, 教授 (00155847) ; MONZEN Satoru 弘前大学, 大学院保健学研究科, 助教 (20514136) ; YOSHINO Hironori 弘前大学, 大学院保健学研究科, 助教 (10583734)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 放射線被ばく / 血清中microRNA / バイオマーカー

Outline of Final Research Achievements

The present study aimed to identify increasing serum microRNAs within three days using mice exposed to ionizing radiation (IR). The sera of mice were collected at 24 h, 48 h, and 72 h after exposure to a 7 Gy dose of X-ray irradiation. The microRNAs extracted from the sera were labeled with Cyanine-3 (Cy3) fluorescent dye, and the profiles of microRNA expression in the sera were performed using the Agilent SurePrint G3 Mouse microRNA microarray slide and Cy3-labeled microRNAs. Fifty-eight microRNAs were found to be expressed differentially in the sera of IR-exposed mice (7 Gy), with a magnitude of more than 1.5-fold change as compared to the sera of IR-unexposed mice (0 Gy). Among them, miR-375-3p increased 1.9-, 5.4-, and 12.7-fold change at 24 h, 48 h, and 72 h after exposure to 7 Gy dose of X-ray irradiation, respectively. This suggests that miR-375-3p in serum has the potential to be used as a biomarker to identify exposure to IR.

Free Research Field

分子生物学