Possiblity of fatty acid binding protein as a diagnostic and therapeutic target in glioma

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670274
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Yamaguchi University
• Principal Investigator: OWADA Yuji 山口大学, 医学(系)研究科(研究院), 教授 (20292211)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Michiyasu 山口大学, 大学院医学系研究科, 教授 (80196873)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: FABP / 脳腫瘍 / 診断マーカー / 増殖

Outline of Final Research Achievements

In this study, we tried to develop the sensitive diagnostic tool for glioblastoma focusing on fatty acid binding protein (FABP7), and examined the possibility as a regulator of glioma proliferation. We firstly established the sensitive ELISA assay system to detect FABP7 and measured its concentration in the cerebro-spinal fluid (CSF) samples from the patient suffering from glioma. As a result, FABP7 concentration in the CSF was markedly elevated in the glioblastoma patients compared with healthy controls. In the pathological examination in glioma samples, levels of FABP7 expression were significantly correlated with their malignancy grade. Furthermore, suppression of FABP7 expression in glioma stem cell lines by siRNA transfection resulted in the decreased proliferation.

Free Research Field

組織学、解剖学、分子生物学