2014 Fiscal Year Final Research Report
Generation and clinical application of low-molecular-weight functional domain proteins.
| Project/Area Number |
25670280
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OYAMA Hiroyuki 神戸薬科大学, 薬学部, 助教 (80572966)
MORITA Izumi 神戸薬科大学, 薬学部, 助手 (20299085)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 進化分子工学 |
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| Outline of Final Research Achievements |
Although antibodies are essential for trace characterization of diagnostic biomarkers, their high molecular weight and low expression levels in E.coli make it difficult to improving their functions by genetic engineering. To overcome these limitations, we planned generation of low-molecular-weight functional domain proteins. First, we made a molecular library, each member of which has the VH domain of an antibody as a fundamental structure and a randomized complementarity-determining region 3 loop, but any practical species could not be isolated. Then, another library based on the streptavidin monomer was produced. Three loop structures, which were supposed to contact with steroids by in silico modeling, were selected as the target of randomization. This library provided the species that were evaluated to had gained the ability to recognize and bind estradiol or cortisol.
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| Free Research Field |
医歯薬学
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