2014 Fiscal Year Final Research Report
Application of mRNA expression analysis for human blood identification to degenerated samples that were false-negative by immunochromatography
| Project/Area Number |
25670347
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSUE Aya 福岡大学, 医学部, 講師 (70309920)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 個人識別 / 血液証明法 / ヒト特異性 / 血液特異性 / mRNA / glycophorin遺伝子 / beta-spectrin遺伝子 / beta-hemoglobin遺伝子 |
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| Outline of Final Research Achievements |
We often encounter cases in which methamphetamine (MA) mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. We investigated the application of mRNA expression analysis for those samples that showed a false-negative with immunochromatography as an alternative approach that does not depend on the antigen-antibody reaction. We used real-time PCR to examine the expression levels of blood markers such as glycophorin A (GYPA), beta-spectrin (SPTB) and beta-hemoglobin (HBB). HBB was the only marker detected in blood specifically, and GYPA was useful in determining human specificity. HBB showed good detection sensitivity, and was detectable from older blood stains. HBB was exclusively detectable in MA-mixed blood stains. Detergents and disinfectants did not significantly influence the mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography.
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| Free Research Field |
法医学
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