2013 Fiscal Year Final Research Report
Identification of noncoding RNAs that regulate macrophage dynamics in chronic inflammation
| Project/Area Number |
25670381
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Cardiovascular medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MANABE Ichiro 東京大学, 医学部附属病院, 講師 (70359628)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIU Katsuhito 東京大学, 医学部附属病院, 特任助教 (30422306)
MATSUMOTO Sahohime 東京大学, 医学部附属病院, 特任助教 (80570184)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Keywords | 非翻訳RNA / マクロファージ |
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| Research Abstract |
Macrophages are the key effector cells in chronic inflammatory processes in cardiovascular disease. They alter their phenotypes and functions during the processes. This study aimed to identify the noncoding RNAs that control dynamic changes in macrophage functions. We identified more than 1,000 novel long noncoding RNAs (lncRNAs0 expressed in macrophages. We also found that some of them bind to the PU.1 transcriptional regulatory complexes. Among them two lncRNAs are induced by LPS in cultured macrophages. They are also induced by tissue injury in macrophages in in vivo settings. Knocking down the lncRNAs altered the response of macrophages to LPS, suggesting they control macrophage functions.
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