Three-dimensional reconstruction of bioartificial heart by reprogrammed human cardiomyocytes

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670389
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Okayama University
• Principal Investigator: OH HIDEMASA 岡山大学, 大学病院, 教授 (50372579)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: バイオ人工心臓 / 心臓内幹細胞 / 細胞外マトリックス

Outline of Final Research Achievements

The shortage of donors for heart transplantation has become a critical issue to treat the patients with end-stage of cardiac failure. We have decellularized the rat whole heart by using pulsatile perfusion apparatus. Reprogrammed human cardiomyocytes were mixed with endothelial progenitor cells in culture and those were perfused to recellularize the acellular construct. Following 8 to 10 days in continuous perfusion, reseeded cells were engrafted onto the matrix to reconstruct the entire vascular beds and myocardium ex vivo.

Free Research Field

循環器内科学