Functional Analysis of Muscle Specific NAD/NADH Dehydrogenase, and Establishment as a Biomarker for Cardiac Dysfunction.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670392
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Kyushu University
• Principal Investigator: SUNAGAWA Kenji 九州大学, 医学(系)研究科(研究院), 教授 (50163043)
• Co-Investigator(Kenkyū-buntansha): ARAI Shinobu 九州大学, 病院学術研究員 (30529970)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: カルシウム / 筋肥大 / 心肥大

Outline of Final Research Achievements

DHRS7C (Dehydrogenase/Reductase (SDR Family) Member 7C) is a novel unknown molecule expressed specifically in heart and skeletal muscle. We found DHRS7c correlates strongly with left ventricular (LV) function from . We herein aimed to investigate the role of DHRS7C in the development of heart failure and to establish as a diagnostic marker of LV dysfunction. We found that DHRS7C is a major regulator of cellular calcium homeostasis, locating in sarcoplasmic reticulum. Moreover, we also found the expression of DHRS7C is regulated by hypoxia inducible factor (HIF-1). We established a suitable condition of antibodies and protocol for ELISA. We found further determination of serum pretreatment condition is necessary in order to obtain higher sensitivity and to reduce background. We are working for measuring the level of DHRS7C in serum from healthy volunteer and heart failure patients.

Free Research Field

循環器内科学