Role of megalin-dependent and -independent pathways in renal protein reabsorption

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670409
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Kyoto University
• Principal Investigator: MORI Kiyoshi 京都大学, 医学(系)研究科(研究院), 准教授 (60343232)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: メガリン / 再吸収 / アルブミン / 尿細管 / 集合管

Outline of Final Research Achievements

We generated drug-inducible, proximal tubule-specific megalin knockout mice. Using these mice, we could visualize megalin-independent protein reabsorption in collecting ducts. Reabsorption of retinol-binding protein, a low molecular weight protein, was almost completed at S1-S2 segments of renal cortex. Quantitative contribution of megalin(+) S3 segment (in outer stripe of outer medulla) and megalin(-) collecting ducts for protein reabsorption was relatively very small. Further analysis is required to investigate whether protein reabsorption at collecting ducts elicits inflammatory signals in those cells.

Free Research Field

腎臓内科学