Basic research of new epigenetic marker, H4K20ac

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670410
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Osaka University
• Principal Investigator: Kaimori Jun-Ya 大阪大学, 医学(系)研究科(研究院), 寄附講座准教授 (70527697)
• Co-Investigator(Kenkyū-buntansha): ISAKA Yoshitaka 大阪大学, 大学院医学系研究科, 教授 (00379166)
• Co-Investigator(Renkei-kenkyūsha): TAKAHARA Shiro 大阪大学, 大学院医学系研究科, 寄附講座教授 (70179547)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: epigenetics / epigenome / histone modification / gene expression

Outline of Final Research Achievements

By suing mass spectmetory analyses, We identified H4K20ac, which was discovered only in plant cells. To understand the function of H4 lysine 20 acetylation (H4K20ac), we have developed a specific monoclonal antibody and performed ChIP-seq analysis using HeLa-S3 cells. H4K20ac was enriched around the transcription start sites (TSSs) of minimally expressed genes and in the gene body of expressed genes, in contrast to most histone acetylation being enriched around the TSSs of expressed genes. The distribution of H4K20ac showed little correlation with known histone modifications, including histone H3 methylations.

Free Research Field

腎臓内科