2015 Fiscal Year Final Research Report
Creating the ureteric bud with a nephron-inducing potential
| Project/Area Number |
25670413
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 腎臓発生 / 尿管芽 / ネフロン |
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| Outline of Final Research Achievements |
We previously succeeded in generating the kidney tissues in vitro by inducing nephron progenitors from pluripotent stem cells. However, the kidney is formed through the mutual interactions between the nephron progenitors and the ureteric bud. Therefore this project aims at differentiating uncommitted precursors toward the functional ureteric bud that possesses a nephron-inducing potential. We utilized genetically modified mice and identified several signature genes that represent the developmental stages during ureteric bud formation. By using these markers, we tried to induce the ureteric bud from the mesoderm in early stage embryos. In addition, we established the embryonic stem cells from the mice described above, and are trying to induce these cells toward the ureteric bud lineage.
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| Free Research Field |
腎臓発生
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