2013 Fiscal Year Final Research Report
Elucidation of mechanisms of myelodysplastic syndrome progression by RNAi screening.
| Project/Area Number |
25670445
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
KUROKAWA Mineo 東京大学, 医学部附属病院, 教授 (80312320)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KATAOKA Keisuke 東京大学, 医学部附属病院, 特任助教 (90631383)
|
|---|
| Project Period (FY) |
2013-04-01 – 2014-03-31
|
| Keywords | 骨髄異形成症候群 / 治療抵抗性 / RNAiスクリーニング / 網羅的ゲノム解析 |
|---|
| Research Abstract |
The aim of this study is to elucidate the mechanism of resistance to treatment in myelodysplastic syndrome (MDS) patients by using RNA-interference technologies. We employed the small-hairpin RNA plasmid library from the Decipher (Cellcta, Mountain View, CA, USA) and transduced into MDS-derived human cell lines. Transduced cell lines were treated with azacitidine, a first-line drug for MDS patients, for 30 days. Then, we isolated genomic DNA and detected specific barcode sequences for each shRNA plasmid with next-generation sequencing. By comparing the clonal composition with or without azacitidine treatment, we identified 158 candidate genes that may induce the azacitidine resistance in MDS.
|
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells2013
Author(s)
Goyama S, Schibler J, Cunningham L, Zhang Y, Rao Y, Nishimoto N, Nakagawa M, Olsson A, Wunderlich M, Link KA, Mizukawa B, Grimes HL, Kurokawa M, Liu PP, Huang G, and Mulloy JC.
-
Journal Title
J Clin Invest.
Volume: 123
Pages: 3876-88
-
-
-
-
-
-
-
-
-
-
-
