Identification of cytokines responsible for the development of immune-mediated bone marrow failure using gene expression analyses of patients with aplastic anemia

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670448
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Kanazawa University
• Principal Investigator: NAKAO Shinji 金沢大学, 医学系, 教授 (70217660)
• Co-Investigator(Renkei-kenkyūsha): OGAWA Seishi 京都大学, 医学(系)研究科(研究院), 教授 (60292900)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 再生不良性貧血 / 造血抑制性サイトカイン / インターロイキン１０ / CXCL12 / クローン性造血

Outline of Final Research Achievements

In an attempt to identify cytokines responsible for the development of immune-mediated bone marrow failure, we determined gene expression profiles of peripheral blood leukocytes from eight untreated patients with non-severe aplastic anemia who had not been transfused. Expressions of several genes associated with erythropoiesis as well as signaling pathways involved in the expression of IL10 and CXCL12 were found to be augmented. Whole exome sequencing of peripheral blood leukocytes from 12 aplastic anemia patients who obtained a complete remission after immunosuppressive therapy revealed somatic mutations of genes related to hematopoietic stem cell quiescence such as PEG3 and SMARCA2.

Free Research Field

血液内科学