2014 Fiscal Year Final Research Report
Construction of an analysis system for antigen-specific immune response in human autoimmunity
Project/Area Number |
25670459
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Collagenous pathology/Allergology
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Research Institution | The University of Tokyo |
Principal Investigator |
FUJIO KEISHI 東京大学, 医学部附属病院, 講師 (70401114)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Keywords | 自己抗原 / T細胞レセプター / 次世代シークエンス / 関節リウマチ |
Outline of Final Research Achievements |
The aim of this project is to analyze the kinetics of autoreactive T cells in autoimmunity using T cell receptor. We performed single-cell transcriptome analysis and TCR repertoire analysis using next-generation sequence in peripheral blood and synovium CD4+ T cells in rheumatoid arthritis (RA). Expanded clones in the joint belonged to CXCR3-CCR6-CXCR5- fraction in the peripheral blood, and they aquired CXCR4 expression and senescent phenotype in the synovium. In the multicolor flowcytometory-based clinical database revealed that CXCR4+ memory T cells are closely associated with disease activity in RA. This research clarified the previously unknown kinetics of pathgenic CD4+ T cell clone in RA.
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Free Research Field |
アレルギー・リウマチ学
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