Basic study for development of a novel anti-tuberculosis vaccine using magnetite nanoparticles

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670462
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Infectious disease medicine
• Research Institution: Tohoku University
• Principal Investigator: KAWAKAMI KAZUYOSHI 東北大学, 医学(系)研究科(研究院), 教授 (10253973)
• Co-Investigator(Kenkyū-buntansha): ISHII Keiko 東北大学, 大学院医学系研究科, 准教授 (00291253) ; TAKAMI Seiichi 東北大学, 多元物質科学研究所, 准教授 (40311550) ; TOGASHI Takanari 山形大学, 理学部, 研究支援者 (80510122)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ナノ粒子ワクチン

Outline of Final Research Achievements

Tuberculosis is still an important health problem in the world, and development of a more effective vaccine has been desired. Recently, a novel approach using a nanoparticle technology has been conducted for development of vaccine. In this study, we performed a basic investigation to develop a new vaccine using magnetite nanoparticles. We succeeded in binding green fluorescence protein (GFP) to magnetite nanoparticles by introducing tandemly-arranged 6 arginines into this protein. In addition, we demonstrated that GFP-bound nanoparticles were incorporated by dendritic cells in the lungs of mice after intratracheal inoculation, which led to the immune response to this protein. Development of a novel vaccine may be expected by introducing vaccine antigen into the magnetite nanoparticles.

Free Research Field

感染症内科