2015 Fiscal Year Final Research Report
Novel diagnostic method for infectious disease using next generation sequencing
| Project/Area Number |
25670468
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HAYASHI Tetsuya 九州大学, 大学院医学研究院, 教授 (10173014)
OKAYAMA Akihiko 宮崎大学, 医学部, 教授 (70204047)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | メタゲノム / 感染症 / 未培養 |
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| Outline of Final Research Achievements |
In the diagnosis and treatment of infectious diseases, the time that required for identification of the pathogen from patients, directly faces to the problem of the starting point for appropriate treatment and affects on patient prognosis. The exhaustive detection method for pathogens using the next generation sequencers without cultivation for isolation was developed. In the case of analyses such as blood, the current DNA purification method contaminates the large amount of host-derived DNA. It interferes to get enough of the sequence information of the pathogen of interest. Therefore, we developed a DNA purification method and identification method for efficiently acquiring information of the pathogen.
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| Free Research Field |
細菌ゲノム
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