• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Molecular analysis of fetal hematopoiesis using culture system with ES cells

Research Project

  • PDF
Project/Area Number 25670489
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Embryonic/Neonatal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

EBIHARA Yasuhiro  東京大学, 医科学研究所, 助教 (40302608)

Co-Investigator(Kenkyū-buntansha) MOCHIZUKI Shinji  東京大学, 医科学研究所, 特任助教 (90349473)
OTSU Makoto  東京大学, 医科学研究所, 准教授 (30361330)
Project Period (FY) 2013-04-01 – 2014-03-31
Keywords胎児医学 / ES細胞
Research Abstract

We developed the culture system to generate human hematopoietic stem/progenitor cells from human embryonic stem cells (hESCs) more physiologically by the coculture with autologous stromal cells derived from hESCs. In this study, we analyzed fetal hematopoiesis using our culture system.
We revealed that the established stromal cells were derived from mesodermal mesenchymal stem cells (MSCs). Human ESC-derived MSCs described above retained the ability to support the growth of autologous hESCs similar to MEF. And our system was capable of generating a number of adult-type human hematopoietic stem/progenitor cells. Using our coculture system may be available for further analysis of human fetal hematopoiesis.

  • Research Products

    (25 results)

All 2014 2013 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Impact of sex incompatibility on the outcome of single-unit cord blood transplantation for adult patients with hematological malignancies2014

    • Author(s)
      Konuma T, Kato S, Ooi J, Oiwa-Monna M, Ebihara Y, Mochizuki S, Yuji K, Ohno N, Kawamata T, Jo N, Yokoyama K, Uchimaru K, Tojo A, Takahashi S.
    • Journal Title

      Bone Marrow Transplan

      Volume: (In press)

    • DOI

      10.1038/bmt.2014.10.

    • Peer Reviewed
  • [Journal Article] Allogeneic stem cell transplantation for patients with acute myeloid leukemia developing from severe congenital neutropenia2014

    • Author(s)
      Ebihara Y, Ishikawa K, Mochizuki S, Tanaka R, Manabe A, Iseki T, Maekawa T, Tsuji K.
    • Journal Title

      Br J Haematol

      Volume: 164 Pages: 451-464

    • DOI

      10.1111/bjh.12638

    • Peer Reviewed
  • [Journal Article] Effect of ABO blood group incompatibility on the outcome of single-unit cord blood transplantation following myeloablative conditioning2014

    • Author(s)
      Konuma T, Kato S, Ooi J, Oiwa-Monna M, Ebihara Y, Mochizuki S, Yuji K, Ohno N, Kawamata T, Jo N, Yokoyama K, Uchimaru K, Tojo A, Takahashi S.
    • Journal Title

      Biol Blood Marrow Transplant

      Volume: 20(4) Pages: 577-81

    • DOI

      10.1016/j.bbmt.2013.12.563.

    • Peer Reviewed
  • [Journal Article] Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function2014

    • Author(s)
      Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Z, Takubo K, Suda T, Oda H, Inaba T, Honda H.
    • Journal Title

      PLoS One

      Volume: 9(1) Pages: e87425

    • DOI

      10.1371/journal.pone.0087425.

    • Peer Reviewed
  • [Journal Article] Pneumothorax in an early phase after allogeneic hematopoietic stem cell transplantation2013

    • Author(s)
      Ebihara Y, Yamamoto S, Mochizuki S, Tsukada M, Taya Y, Kawakita T, Kato S, Ooi J, Takahashi S, Tojo A, Tsuji K.
    • Journal Title

      Hematol Rep

      Volume: 28 Pages: 34-35

    • DOI

      10.4081/hr.2013.e10

    • Peer Reviewed
  • [Journal Article] Unusual extramedullary relapse after haploidentical bone marrow transplantation in a patient with acute lymphoblastic leukemia2013

    • Author(s)
      Ebihara Y, Yamamoto S, Mochizuki S, Tsukada M, Taya Y, Sato A, Kawakita T, Kato S, Ooi J, Takahashi S, Tojo A, Tsuji K.
    • Journal Title

      J Blood Disorders Transf

      Volume: 4(5) Pages: 1000155

    • DOI

      10.4172/2155-9864.1000155

    • Peer Reviewed
  • [Journal Article] Single-unit cord blood transplantation after granulocyte colony-stimulating factor-combined myeloablative conditioning for myeloid malignancies not in remission2013

    • Author(s)
      Konuma T, Kato S, Ooi J, Oiwa-Monna M, Ebihara Y, Mochizuki S, Yuji K, Ohno N, Kawamata T, Jo N, Yokoyama K, Uchimaru K, Asano S, Tojo A, Takahashi S.
    • Journal Title

      Biol Blood Marrow Transplant

      Volume: 20(3) Pages: 396-401

    • DOI

      10.1016/j.bbmt.2013.12.555.

    • Peer Reviewed
  • [Journal Article] Generation of engraftable hematopoietic stem cells from induced pluripotent stem cells by way of teratoma formation2013

    • Author(s)
      Suzuki N, Yamazaki S, Yamaguchi T, Okabe M, Masaki H, Takaki S, Otsu M, Nakauchi H.
    • Journal Title

      Mol Ther.

      Volume: 21(7) Pages: 1424-31

    • DOI

      10.1038/mt.2013.71.

    • Peer Reviewed
  • [Journal Article] Top-down motif engineering of a cytokine receptor for directing ex vivo expansion of hematopoietic stem cells2013

    • Author(s)
      Saka K, Kawahara M, Teng J, Otsu M, Nakauchi H, Nagamune T.
    • Journal Title

      Journal of biotechnology

      Volume: 168(4) Pages: 659-65

    • DOI

      10.1016/j.jbiotec.2013.09.012.

    • Peer Reviewed
  • [Journal Article] Generation of Col2a1-EGFP iPS cells for monitoring chondrogenic differentiation2013

    • Author(s)
      Saito T, Yano F, Mori D, Ohba S, Hojo H, Otsu M, Eto K, Nakauchi H, Tanaka S, Chung UI, Kawaguchi H.
    • Journal Title

      PLoS One

      Volume: 8(9) Pages: e74137

    • DOI

      10.1371/journal.pone.0074137.

    • Peer Reviewed
  • [Journal Article] Profiling of microRNA in human and mouse ES and iPS cells reveals overlapping but distinct microRNA expression patterns2013

    • Author(s)
      Razak SR, Ueno K, Takayama N, Nariai N, Nagasaki M, Saito R, Koso H, Lai CY, Murakami M, Tsuji K, Michiue T, Nakauchi H, Otsu M, Watanabe S.
    • Journal Title

      PLoS One

      Volume: 8(9) Pages: e73532

    • DOI

      10.1371/journal.pone.0073532.

    • Peer Reviewed
  • [Journal Article] Adipose Natural Regulatory B Cells Negatively Control Adipose Tissue Inflammation2013

    • Author(s)
      Nishimura S, Manabe I, Takaki S, Nagasaki M, Otsu M, Yamashita H, Sugita J, Yoshimura K, Eto K, Komuro I, Kadowaki T, Nagai R..
    • Journal Title

      Cell metabolism

      Volume: 18(5) Pages: 759-766

    • DOI

      10.1016/j.cmet.2013.09.017

    • Peer Reviewed
  • [Journal Article] Stem cell therapy : an exercise in patience and prudence. Philosophical transactions of the Royal Society of London Series B2013

    • Author(s)
      Lin HT, Otsu M, Nakauchi H.
    • Journal Title

      Biological sciences

      Volume: 368(1609) Pages: 20110334

    • DOI

      10.1098/rstb.2011.0334.

    • Peer Reviewed
  • [Journal Article] ACTN1 mutations cause congenital macrothrombocytopenia2013

    • Author(s)
      Kunishima S, Okuno Y, Yoshida K, Shiraishi Y, Sanada M, Muramatsu H, Chiba K, Tanaka H, Miyazaki K, Sakai M, Ohtake M, Kobayashi R, Iguchi A, Niimi G, Otsu M, Takahashi Y, Miyano S, Saito H, Kojima S, Ogawa S.
    • Journal Title

      American journal of human genetics

      Volume: 92(3) Pages: 431-8

    • DOI

      10.1016/j.ajhg.2013.01.015.

    • Peer Reviewed
  • [Journal Article] Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms2013

    • Author(s)
      Kon A, Shih LY, Minamino M, Sanada M, Shiraishi Y, Nagata Y, Yoshida K, Okuno Y, Bando M, Nakato R, Ishikawa S, Sato-Otsubo A, Nagae G, Nishimoto A, Haferlach C, Nowak D, Sato Y, Alpermann T, Nagasaki M, Shimamura T, Tanaka H, Chiba K, Yamamoto R, Yamaguchi T, Otsu M, Obara N, Sakata-Yanagimoto M, Nakamaki T, Ishiyama K, Nolte F, Hofmann WK, Miyawaki S, Chiba S, Mori H, Nakauchi H, Koeffler HP, Aburatani H, Haferlach T, Shirahige K, Miyano S, Ogawa S.
    • Journal Title

      Nature genetics

      Volume: 45(10) Pages: 1232-7

    • DOI

      10.1038/ng.2731.

    • Peer Reviewed
  • [Journal Article] Immortalization of Erythroblasts by c-MYC and BCL-XL Enables Large-Scale Erythrocyte Production from Human Pluripotent Stem Cells2013

    • Author(s)
      Hirose S, Takayama N, Nakamura S, Nagasawa K, Ochi K, Hirata S, Yamazaki S, Yamaguchi T, Otsu M, Sano S, Takahashi N, Sawaguchi A, Ito M, Kato T, Nakauchi H, Eto K.
    • Journal Title

      Stem cell reports

      Volume: 1(6) Pages: 499-508

    • DOI

      10.1016/j.stemcr.2013.10.010.

    • Peer Reviewed
  • [Journal Article] Congenital amegakaryocytic thrombocytopenia iPS cells exhibit defective MPL-mediated signaling2013

    • Author(s)
      Hirata S, Takayama N, Jono-Ohnishi R, Endo H, Nakamura S, Dohda T, Nishi M, Hamazaki Y, Ishii E, Kaneko S, Otsu M, Nakauchi H, Kunishima S, Eto K.
    • Journal Title

      The Journal of clinical investigation

      Volume: 123(9) Pages: 3802-14

    • DOI

      10.1172/JCI64721.

    • Peer Reviewed
  • [Journal Article] Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method2013

    • Author(s)
      Hamanaka S, Ooehara J, Morita Y, Ema H, Takahashi S, Miyawaki A, Otsu M, Yamaguchi T, Onodera M, Nakauchi H.
    • Journal Title

      Biochemical and biophysical research communications

      Volume: 435(4) Pages: 586-91

    • DOI

      10.1016/j.bbrc.2013.05.017.

    • Peer Reviewed
  • [Presentation] 自己骨髄由来間葉系幹細胞を用いた血友病関節症に対する治療の可能性2013

    • Author(s)
      海老原康博, 望月慎史, 大津真, 竹谷英之, 辻浩一郎
    • Organizer
      第55回日本小児血液・がん学会学術集会
    • Place of Presentation
      ヒルトン福岡シーホーク, 福岡
    • Year and Date
      20131129-1201
  • [Presentation] 本邦におけるボルテゾミブ併用化学療法を行った小児再発難治性急性リンパ性白血病 24例の後方視的検討2013

    • Author(s)
      望月慎史, 小川千登世, 佐野秀樹, 木下明俊, 篠田邦大, 宮下恵実子, 吉原宏樹, 井口晶裕, 吉田咲子, 西川拓朗, 西眞範, 豊田秀実, 熊本忠史, 中村和洋, 西内律雄, 菊田敦 , 後藤裕明, 足立壮一, 水谷修紀
    • Organizer
      第55回日本小児血液・がん学会学術集会
    • Place of Presentation
      ヒルトン福岡シーホーク, 福岡
    • Year and Date
      20131129-1201
  • [Presentation] ヒトiPS由来肥満細胞の機能解析2013

    • Author(s)
      五十嵐晃, 熊谷智明, 平井博之, 永田欽也, 花田佐智代, 海老原康博, 辻浩一郎
    • Organizer
      第63回日本アレルギー学会
    • Place of Presentation
      ホテルニューオータニ, 東京
    • Year and Date
      20131128-30
  • [Presentation] Drug screening for the 8p11 myeloproliferative syndrome using patient iPS cells2013

    • Author(s)
      Mai Nanya, Shohei Yamamoto, Yasuhiro Ebihara, Shinji Mochizuki, Makoto Otsu, Minoru Tozuka, Hiromitsu Nakauchi, Kohichiro Tsuji
    • Organizer
      第75回日本血液学会
    • Place of Presentation
      ロイトン札幌, 札幌
    • Year and Date
      20131011-13
  • [Presentation] Generation and analysis of a novel model for CMML with acquired expression of CBL Q367P2013

    • Author(s)
      Yuichiro Nakata,Takeshi Ueda, Norimasa Yamasaki,Akiko Nagamachi, Keiyo Takubo,Yasuhiro Ebihara, Masashi Sanada, Seishi Ogawa, Kohichiro Tsuji,Toshio Suda,Toshiya Inaba, Hiroaki Honda
    • Organizer
      第75回日本血液学会
    • Year and Date
      20131011-13
  • [Presentation] 重症先天性好中球減少症患者由来のiPS細胞の樹立とその分子生物学的解析2013

    • Author(s)
      海老原康博, 平本貴史, 山本将平, 望月慎史, 辻浩一郎, 溝口洋子, 中村和洋, 小林正夫
    • Organizer
      第116回日本小児科学会
    • Place of Presentation
      広島国際会議場, 広島
    • Year and Date
      20130419-21
  • [Remarks] 東京大学医科学研究所 幹細胞治療研究センター幹細胞プロセシング分野

    • URL

      http://stemcell-u-tokyo.org/scp/

URL: 

Published: 2015-06-25  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi