An application of imaging mass spectrometry to the analysis of human placental villous structure –In search of etiology-specific biopolymer-

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670490
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: HIROAKI ITOH 浜松医科大学, 医学部附属病院, 教授 (70263085)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Kazunao 浜松医科大学, 医学部附属病院, 講師 (50456571)
• Co-Investigator(Renkei-kenkyūsha): HAYASAKA Takahiro 浜松医科大学, 医学部, 助教 (90415927)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 胎盤 / 質量顕微鏡法 / 妊娠 / 胎児発育不全 / 妊娠高血圧症候群 / 絨毛 / 病理

Outline of Final Research Achievements

A matrix-assisted laser desorption ionization (MALDI)-based mass spectrometer provides clear two-dimensional molecular identification with highly sensitive mass spectrometry from mixtures of ions generated on tissue surfaces. We applied this technology to the molecular identification of phospholipids in the human term placenta and found that placental some of pathological findings were associated with the distribution pattern of sphingomyelin (d18:1/16:0) and phosphatidylcholine (16:0/20:4) in stem and terminal villi. The findings suggested that these biopolymers may be involved in the maintenance and/or pathological changes placental villus structures.

Free Research Field

周産期医学,　DOHaD