ナノ粒子化したチタン酸化物を用いた新規放射線増感療法

2015 Fiscal Year Annual Research Report

• Project/Area Number: 25670535
• Research Institution: Kobe University
• Principal Investigator: 佐々木 良平 神戸大学, 医学部附属病院, 教授 (30346267)
• Co-Investigator(Kenkyū-buntansha): 荻野 千秋 神戸大学, 工学(系)研究科(研究院), 准教授 (00313693) ; 佐藤 和好 群馬大学, その他部局等, 助教 (40437299) ; 沼子 千弥 千葉大学, 理学(系)研究科(研究院), 准教授 (80284280) ; 中山 雅央 神戸大学, 医学(系)研究科(研究院), 研究員 (60582004) ; 赤坂 浩亮 神戸大学, 医学部附属病院, 助教 (20707161)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 放射線療法 / ナノ粒子

Outline of Annual Research Achievements

本研究では、過酸化チタンナノ粒子による放射線増感効果の有効性を明らかにし、これまでにない放射線増感療法を開発し、膵臓癌などの難治癌に対する新たな治療戦略を提案することである。具体的には、Cell-free system（無機物質：ナノ粒子のみの環境）でのヒドロキシラジカル等のReactive Oxygen Species（ROS）生成メカニズムが過酸化チタンナノ粒子の表面構造に大きく依存することが判明した。細胞実験（有機物質環境下）ではγH2AXで確認できるDNA fociの発生細胞数が、過酸化チタンナノ粒子を作用させた後で放射線照射をした群で有意に上昇していた。また、in vivo 環境でも放射線増感が確認された。チタン酸化物ナノ粒子の毒性評価は採血や実験動物の体重の変化から判定したが、顕著な毒性は確認できなかった。過酸化チタンナノ粒子の尾静脈からの投与では腫瘍と肝臓により効率に集積する実験結果を得ており、今後の研究に重要な知見と考えられた。

Research Products

• [Journal Article] FDG-PET/contrast-enhanced CT as a post-treatment tool in head and neck squamous cell carcinoma: comparison with FDG-PET/non-contrast-enhanced CT and contrast-enhanced CT. (2016)
  • Author(s): Suenaga Y, Kitajima K, Ishihara T, Sasaki R, Otsuki N, Nibu KI, Minamikawa T, Kiyota N, Sugimura K.
  • Journal Title: Eur Radiol. Volume: 26(4) Pages: 1018-1030
  • DOI: 10.1007/s00330-015-3902-1.
  • Peer Reviewed
• [Journal Article] 7.Suenaga Y, Kitajima K, Kanda T, Otsuki N, Nibu KI, Sasaki R, Itoh T, Sugimura K. [(18)F]-FDG PET/CT imaging for detection of nodal metastases in patients with squamous cell carcinoma of the pharynx and larynx: comparison with CT. (2016)
  • Author(s): Suenaga Y, Kitajima K, Kanda T, Otsuki N, Nibu KI, Sasaki R, Itoh T, Sugimura K.
  • Journal Title: Jpn J Radiol. Volume: 34(3) Pages: 203-210
  • DOI: 10.1007/s11604-015-0510-6.
  • Peer Reviewed
• [Journal Article] Concomitant chemoradiotherapy for advanced squamous cell carcinoma of temporal bone (2016)
  • Author(s): Shinomiya H, Hasegawa S, Yamashita D, Ejima Y, Kenji Y, Otsuki N, Kiyota N, Sakakibara S, Nomura T, Hashikawa K, Kohmura E, Sasaki R, Nibu KI.
  • Journal Title: Head Neck. Volume: 38 Suppl 1 Pages: E949-953
  • DOI: 10.1002/hed.24133.
  • Peer Reviewed
• [Journal Article] Particle therapy for clinically diagnosed stage I lung cancer: comparison with pathologically proven non-small cell lung cancer. (2015)
  • Author(s): Fujii O, Demizu Y, Hashimoto N, Takagi M, Terashima K, Mima M, Jin D, Nagano F, Katsui K, Okimoto T, Iwata H, Niwa Y, Yamashita T, Akagi T, Daimon T, Sasaki R, Hishikawa Y, Abe M, Murakami M, Fuwa N.
  • Journal Title: Acta Oncol. Volume: 54(3): Pages: 315-321
  • DOI: 10.3109/0284186X.2014.974828.
  • Peer Reviewed
• [Journal Article] The current status and future of radiotherapy for spinal bone metastases. (2015)
  • Author(s): Ejima Y, Matsuo Y, Sasaki R.
  • Journal Title: J Orthop Sci. Volume: 20(4) Pages: 585-592
  • DOI: 10.1007/s00776-015-0720-x.
  • Peer Reviewed
• [Journal Article] Assessment of Parametrial Response by Growth Pattern in Patients With International Federation of Gynecology and Obstetrics Stage IIB and IIIB Cervical Cancer: Analysis of Patients From a Prospective, Multicenter Trial (EMBRACE). (2015)
  • Author(s): Yoshida K, Jastaniyah N, Sturdza A, Lindegaard J, Segedin B, Mahantshetty U, Rai B, Jürgenliemk-Schulz I, Haie-Meder C, Sasaki R, Pötter R.
  • Journal Title: Int J Radiat Oncol Biol Phys. Volume: 93(4) Pages: 788-796
  • DOI: 10.1016/j.ijrobp.2015.08.007.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Compensatory glutamine metabolism promotes glioblastoma resistance to mTOR inhibitor treatment. (2015)
  • Author(s): Tanaka K, Sasayama T, Irino Y, Takata K, Nagashima H, Satoh N, Kyotani K, Mizowaki T, Imahori T, Ejima Y, Masui K, Gini B, Yang H, Hosoda K, Sasaki R, Mischel PS, Kohmura E.
  • Journal Title: J Clin Invest. Volume: 125(4) Pages: 1591-1602
  • DOI: 10.1172/JCI78239
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Hypofractionated Stereotactic radiotherapy Compared with Stereotactic Radiosurgery for brain metastases from Lung Cancer: Experience at a Single Institution (2015)
  • Author(s): "Takeaki Ishihara, Kazunari Yamada Yasuo Ejima, Kenji Yoshida, Daisuke Miyawaki ,Haruka Uezono, Shazrina Sulaiman, Yoshirou Matsuo, Ryo Nishikawa and Ryohei Sasaki
  • Organizer: 57th Annual Meeting of ASTRO
  • Place of Presentation: San Antonio, USA
  • Year and Date: 2015-10-18 – 2015-10-21
  • Int'l Joint Research
• [Presentation] Radiation Induced Hypothyroidism in Squamous Cell Carcinoma of the Head and Neck: a Dose-Volume Histogram Analysis (2015)
  • Author(s): D. Miyawaki, N. Mukumoto, Y. Ejima, K. Yoshida, T. Ishihara, N. Kiyota, M. Saito, N. Otsuki, K. Nibu, R. Sasaki
  • Organizer: European Cancer Congress 2015
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2015-09-25 – 2015-09-29
  • Int'l Joint Research
• [Presentation] Titanium peroxide nanoparticles, as novel radiosensitizers, enhance antitumor efficacy in pancreatic cancer therapy (2015)
  • Author(s): Masao Nakayama, Ryohei Sasaki, Chiaki Ogino, Tsutomu Tanaka, Kenta Morita, Mitsuo Umetsu, Satoshi Ohara, Kazuyoshi Sato, Chiya Numako, Seiichi Takami, Akihiko Kondo
  • Organizer: 15th International Congress of Radiation Research
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2015-05-25 – 2015-05-29
  • Int'l Joint Research
• [Presentation] Radiosensitizor extracted from Spinach, MGDG, Enhanced the Cytotoxic Effect for Pancreatic Cancer Cells when combined with Radiation Treatment (2015)
  • Author(s): Hiroaki Akasaka, Yasuo Ejima, Kenji Yoshida, Daisuke Miyawaki, Takeaki Ishihara, Yoshiyuki Mizushina and Ryohei Sasaki
  • Organizer: 15th International Congress of Radiation Research
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2015-05-25 – 2015-05-29
  • Int'l Joint Research
• [Presentation] Titanium peroxide nanoparticles enhance antitumor efficacy through reactive oxygen species in pancreatic cancer radiation therapy (2015)
  • Author(s): Masao Nakayama, Ryohei Sasaki, Toru Mukohara, Chiaki Ogino, Kenta Morita, Mitsuo Umetsu, Satoshi Ohara, Kazuyoshi Sato, Chiya Numako, Seiichi Takami, Akihiko Kondo
  • Organizer: AACR Annual Meeting 2015
  • Place of Presentation: Philadelphia, US
  • Year and Date: 2015-04-18 – 2015-04-22
  • Int'l Joint Research
• [Book] Non-woven Fabrics (ISBN 978-953-51-2271-5) (2016)
  • Author(s): Ryohei Sasaki, Hiroaki Akasaka, Yusuke Demizu, Sachiko Inubushi, Tianyuan Wang, Takumi Fukumoto.
  • Total Pages: 315 (205-214)
  • Publisher: INTECH
• [Book] 多職種チームで実践する頭頚部がんの化学放射線療法 (2015)
  • Author(s): 丹生健一、佐々木良平、大月直樹、大田史江
  • Total Pages: 272
  • Publisher: 日本看護協会出版会