Development of extracorporeal therapeutic method of liver grafts using siRNA during machine perfusion preservation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670547
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Hokkaido University
• Principal Investigator: TAKETOMI Akinobu 北海道大学, 医学(系)研究科(研究院), 教授 (70363364)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 肝移植 / 機械灌流 / グラフト治療

Outline of Final Research Achievements

We conducted this study to develop an extracorporeal siRNA transfection method during machine perfusion. Because siRNA could not be transfected under hypothermic condition and perfusion fluid without blood components was preferable as a carrier of siRNA, we achieved sub-normothermic oxygenated machine perfusion (20℃, 180min) in mouse liver transplantation model. We performed extracorporeal siRNA transfection on this mouse model, however, siRNA could not be introduced into liver grafts under such perfusion conditions. According to the results of donor siRNA transfection model, it was suggested that siRNA up-take activities of the liver grafts were not maintained at sufficient levels at 20℃.

Free Research Field

消化器外科学