Induction of anti-tumor immune response using a Tissue Engineering technique

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670558
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Fukushima Medical University
• Principal Investigator: GOTOH Mitsukazu 福島県立医科大学, 医学部, 教授 (50162160)
• Co-Investigator(Kenkyū-buntansha): KENJO Akira 福島県立医科大学, 医学部, 准教授 (40305355) ; KIMURA Takashi 福島県立医科大学, 医学部, 助教 (00381369) ; ANAZAWA Takayuki 福島県立医科大学, 医学部, 助教 (90566811) ; TSUCHIYA Takao 福島県立医科大学, 医学部, 博士研究員 (70343390)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 腫瘍免疫 / 徐放物質 / フィブリン / OK-432

Outline of Final Research Achievements

Focusing on scaffold function of fibrin, we carried out research for development of immunotherapy for solid tumors. The microstructure of fibrin was observed with electron microscope to determine the optimal dilution. In macrophage infiltration assay using fibrin containing OK-432, significantly large number of cells were observed in fibrin gel compared to fibrin without OK-432. In the rat portal vein embolization model, we evaluated the extent of liver tissue damage and cell infiltration due to the difference of embolic materials. When embolization was performed using embolic material containing fibrinogen, histological damage of the liver in association with significant number of infiltrating macrophages was observed until 7 days after the embolization. These data together suggested that coexistence of fibrin and OK-432 has affected the local immune environment.

Free Research Field

医学