2014 Fiscal Year Final Research Report
Fluorescent virus-guided capturing of epithelial-mesenchymal transition (EMT)-induced circulating tumor cells (CTCs) for genetic analysis
| Project/Area Number |
25670580
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAGAWA Shunsuke 岡山大学, 大学病院, 准教授 (00362971)
SHIRAKAWA Yasuhiro 岡山大学, 大学院医歯薬学総合研究科, 准教授 (60379774)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 循環癌細胞 / 上皮間葉転換 / テロメラーゼ / アデノウイルス / TGF-beta / GFP / 遺伝子解析 / PCR |
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| Outline of Final Research Achievements |
Molecular-based companion diagnostic tests are being used with increasing frequency to predict their clinical response to various drugs. However, invasive procedures are typically required to obtain tissues for this analysis. Here we established a new approach to capture live circulating tumor cells (CTCs) among millions of peripheral blood leukocytes using a GFP-expressing attenuated adenovirus, in which the telomerase promoter regulates viral replication (TelomeScan). Because current CTC detection strategies mainly depend on epithelial cell surface markers, the presence of heterogeneous populations of CTCs with epithelial and/or mesenchymal characteristics may pose obstacles to the detection of CTCs. Our biological capturing system can image both epithelial and mesenchymal tumor cells with telomerase activities as GFP-positive cells. This fluorescent virus-guided viable CTC capturing method provides a non-invasive alternative to tissue biopsy.
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| Free Research Field |
消化器外科学
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