The role of SIRT1 in cellular senescence and characteristics in brain microvascular endothelial cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670612
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Hokkaido University
• Principal Investigator: HOUKIN Kiyohiro 北海道大学, 大学病院, 教授 (90229146)
• Co-Investigator(Kenkyū-buntansha): NAKAYAMA Naoki 北海道大学, 大学院医学研究科, 講師 (40421961) ; KAZUMATA Ken 北海道大学, 大学病院, 講師 (60634144) ; ABUMIYA Takeo 北海道大学, 大学院医学研究科, 特任助教 (80270726) ; SHICHINOHE Hideo 北海道大学, 大学病院, 助教 (80374479)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 脳微小血管 / 細胞老化 / SIRT1

Outline of Final Research Achievements

The present study was performed with cultured senescent bovine brain endothelial cells (BMEC) with high passage numbers. P15-20 senescent BMEC contained giant cells with increase of β-galactosidase activity and reactive oxygen species. In comparison between P5 BMEC and P 15 BMEC subjected to oxygen-glucose deprivation and reoxygenation, P15 BMEC showed significantly more cell death and apoptosis. P5 BMEC showed significantly more induction of SIRT1 expression. This result suggests that SIRT1 seems to have cellular protective effects and lose its capacity of induction in senescent cells.

Free Research Field

脳血管障害