crosstalk between stress response and the circadian rhythm in cartilage

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670655
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KUBO TOSHIKAZU 京都府立医科大学, 医学(系)研究科(研究院), 教授 (20178031)
• Co-Investigator(Kenkyū-buntansha): ARAI Yuji 京都府立医科大学, 医学研究科, 講師 (50347449) ; NAKAGAWA Shuji 京都府立医科大学, 医学研究科, 助教 (30643382)
• Co-Investigator(Renkei-kenkyūsha): YAGITA Kazuhiro 京都府立医科大学, 医学研究科, 教授 (90324920)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 軟骨 / 概日リズム

Outline of Final Research Achievements

We demonstrated that ‘‘tissue-autonomous’’ circadian rhythm in the articular cartilage and the growth plate. We revealed a robust and extremely long-lasting circadian rhythm in ex vivo culture maintained for over six months from the femoral bone of a Period2::Luciferase mouse. Furthermore, real-time bioluminescence microscopic imaging revealed that the robust circadian rhythms emanated from the articular cartilage and the epiphyseal cartilage within the growth plate of juvenile animals. Stimulation by forskolin or dexamethasone treatment caused type 0 phase resetting, indicating canonical entraining properties of the cartilage circadian clock. We also showed that Per2::Luc activity in the growth plate and the articular cartilage were phase-shifted by Parathyroid hormone via PTH type1 receptor.

Free Research Field

軟骨