Regulatory effects of GABA-A receptor trafficking and/or receptor phosphorylation on anesthetics

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670663
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: Hirosaki University
• Principal Investigator: UENO Shinya 弘前大学, 医学(系)研究科(研究院), 教授 (00312158)
• Co-Investigator(Renkei-kenkyūsha): YAMADA Junko 弘前大学, 大学院医学研究科, 講師 (30334965) ; MIGITA Keisuke 弘前大学, 大学院医学研究科, 助教 (10352262) ; TOMIYAMA Masahiko 弘前大学, 大学院医学研究科, 客員研究員 (40311542)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: extrasynaptic GABA-A 受容体 / 受容体トラフィッキング / 脱リン酸化調節 / 受容体可視化

Outline of Final Research Achievements

The sensitivity to anesthetics was examined using transgenic mice with abnormal distribution of GABA-A receptors. Electrophysiological approach using slice patch clamp preparation demonstrated that anesthetics failed to enhance GABAergic response through extrasynaptic GABA-A receptors. Based on the evaluation of righting reflex, mice showed tolerance of anesthetics. Administration of dephosphorylation inhibitor cause these mice to recover the sensitivity to anesthetics. These result suggest that anesthetic effect could be regulated via trafficking and/or phosphorylation of GABA-A receptors.

Free Research Field

神経科学