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2015 Fiscal Year Annual Research Report

デスフルランはなぜ術後認知機能低下を防ぐのか:高齢者の細胞骨格関連遺伝子の解析

Research Project

Project/Area Number 25670673
Research InstitutionSapporo Medical University

Principal Investigator

山蔭 道明  札幌医科大学, 医学部, 教授 (70285005)

Co-Investigator(Kenkyū-buntansha) 杉野 繁一  東北大学, 大学病院, 助教 (00423765) [Withdrawn]
早瀬 知  札幌医科大学, 医学部, 助教 (20579007)
Project Period (FY) 2013-04-01 – 2016-03-31
Keywords麻酔科学
Outline of Annual Research Achievements

申請者らは本研究を通じて、臨床課題として術前認知機能障害のない65歳以上の患者を対象とし、比較的長時間手術におけるセボフルランおよびデスフルランの術後認知機能障害の発症についての検討を高次脳機能テスト(MMSE)を行うことで実施した。デスフルランで麻酔を受けた群ではセボフルランで麻酔を受けた群と比較してMMSEスコアが良好である傾向が示された。その分子生物学的な機序を探索するため、マウス海馬ニューロンにおける全mRNAの発現変化を一元的に解析した(トランスクリプトーム解析)。その結果、8週齢のマウスでは吸入麻酔薬曝露はRtn4rl2遺伝子が最も発現が増大しLIM-homeodomain family遺伝子群が最も抑制されることが示された。吸入麻酔薬曝露は神経幹細胞の誘導に影響を与える可能性が示唆された。さらに35週齢以降のマウスについての海馬ニューロンにおける全mRNA解析を追加し、週齢による遺伝子発現の変化を検討した。その結果、35週齢のマウスにおいてLIM-homeodomain familyの一つであるLhx9遺伝子が最も発現が増大傾向を示し、Epyc遺伝子が最も抑制された。週齢の差によってLIM-homeodomain familyの発現が大きく異なることは、これまで基礎的に幼若期の吸入麻酔薬曝露による神経炎症だけが良く研究されていた分野であったことに対し、成熟した脳においても別の機序で術後認知機能障害を引き起こしているのではないかと示唆するものである。

  • Research Products

    (10 results)

All 2016 2015

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Effect of sevoflurane anesthesia on the comprehensive mRNA expression profile of the mouse hippocampus2016

    • Author(s)
      Hayase T, Tachibana S, Yamakage M.
    • Journal Title

      Med Gas Res

      Volume: 6 Pages: 1-7

    • Peer Reviewed / Open Access
  • [Journal Article] Efficacy and Safety of a Lidocaine and Ropivacaine Mixture for Scalp Nerve Block and Local Infiltration Anesthesia in Patients Undergoing Awake Craniotomy.2016

    • Author(s)
      Chaki T, Sugino S, Janicki PK, Ishioka Y, Hatakeyama Y, Hayase T, Kaneuchi-Yamashita M, Kohri N, Yamakage M.
    • Journal Title

      J Neurosurg Anesthesiol

      Volume: 28 Pages: 1-5

    • DOI

      10.1097/ANA.0000000000000149

    • Peer Reviewed
  • [Journal Article] Nitrite Reduces Ischemia-Induced Ventricular Arrhythmias by Attenuating Connexin 43 Dephosphorylation in Rats.2016

    • Author(s)
      Maruyama D, Hirata N, Tokinaga Y, Kawaguchi R, Yamakage M.
    • Journal Title

      Anesth Analg

      Volume: 122 Pages: 410-417

    • DOI

      10.1213/ANE.0000000000001063.

    • Peer Reviewed
  • [Journal Article] TACR1 gene polymorphism is associated with gender differences in postoperative nausea and vomiting.2015

    • Author(s)
      Hayase T, Sugino S, Moriya H, Yamakage M.
    • Journal Title

      Anaesthesia

      Volume: 70 Pages: 1148-1159

    • DOI

      10.1111/anae.13082

    • Peer Reviewed
  • [Journal Article] Recovery of postoperative cognitive function in elderly patients after a long duration of desflurane anesthesia: a pilot study.2015

    • Author(s)
      Tachibana S, Hayase T, Osuda M, Kazuma S, Yamakage M.
    • Journal Title

      J Anesth

      Volume: 29 Pages: 627-630

    • DOI

      10.1007/s00540-015-1979-y

    • Peer Reviewed
  • [Presentation] Possible Mechanism of Neuroinflammation Induced by Surgical Procedure in the Mouse Hippocampus Determined by Using Transcriptome Analysis.2015

    • Author(s)
      Tachibana S, Hayase T, Yamakage M.
    • Organizer
      The Annual Meeting of American Society of Anesthesiologists
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-10-24 – 2015-10-28
    • Int'l Joint Research
  • [Presentation] Prediction of Molecular Mechanism of Postoperative Nausea and Vomiting Using Transcriptome Analysis.2015

    • Author(s)
      Hayase T, Tachibana S, Yamakage M.
    • Organizer
      The Annual Meeting of American Society of Anesthesiologists
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-10-24 – 2015-10-28
    • Int'l Joint Research
  • [Presentation] Effects of Propofol and Sevoflurane on Heating-induced Tension Generation of Vascular Smooth Muscle in Rats.2015

    • Author(s)
      Kimizuka M, Takada Y, Tokinlaga Y, Hayashi S, Kazuma S, Azumaguchi R, Yamakage M.
    • Organizer
      The Annual Meeting of American Society of Anesthesiologists
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-10-24 – 2015-10-28
    • Int'l Joint Research
  • [Presentation] Elevation and Suppression of Paraoxon-induced Epileptic Activity in the Rat Hippocampus.2015

    • Author(s)
      Takada Y, Narimatsu E, Takahashi K, Niiya T, Yamakage M.
    • Organizer
      The Annual Meeting of American Society of Anesthesiologists
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-10-24 – 2015-10-28
    • Int'l Joint Research
  • [Presentation] Cardioprotective Effect of Dexmedetomidine Against Ischemia/Reperfusion Injury in Hypertensive Hypertrophied Myocardium.2015

    • Author(s)
      Yoshikawa Y, Hirata N, Kawaguchi R, Miyashita R, Yamakage M.
    • Organizer
      The Annual Meeting of American Society of Anesthesiologists
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-10-24 – 2015-10-28
    • Int'l Joint Research

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Published: 2017-01-06  

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