The investigation of the prostate cancer metastases inhibition method which targets the MRTF mediated signal pathway to cytoskeletal dynamics

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670687
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: MIKI Tsuneharu 京都府立医科大学, 医学(系)研究科(研究院), 教授 (10243239)
• Co-Investigator(Kenkyū-buntansha): KIMURA Yasunori 京都府立医科大学, 医学研究科, 助教 ; KAMOI Kazumi 京都府立医科大学, 医学研究科, 講師 (40295663) ; UEDA Takashi 京都府立医科大学, 医学研究科, 助教 (50601598)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: MRTF-A分子 / 前立腺癌治療 / 細胞骨格 / 癌転移抑制

Outline of Final Research Achievements

We identified that MRTF-A inhibits cancer cell motility by upregulating the various kinds of actin binding proteins in vitro. It was indicated that MRTF-A might be related with tumor-suppressor gene p53 according to the data of MRTF-A promoter assay and knockdown of p53 gene using siRNA in prostate cancer cells. We made adenovirus and retrovirus vectors which overexpress MRTF-A towards prostate cancer cells. Overexpression of MRTF-A in prostate cancer cells reduced cancer cell invasion in culture. Analysis of the effect for tumor cell metastasis in vivo in a mouse model of prostate cancer using these vectors is currently in progress.

Free Research Field

臨床腫瘍学、癌転移