Platelet-induced re-epithelialization and its failure

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670702
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Kanazawa University
• Principal Investigator: FUJIWARA HIROSHI 金沢大学, 医学系, 教授 (30252456)
• Co-Investigator(Kenkyū-buntansha): SATO Yukiyasu 京都大学, 医学(系)研究科(研究院), 助教 (00508236) ; HORIE Akihito 京都大学, 医学(系)研究科(研究院), 助教 (30535836) ; ARAKI Yoshihiko 順天堂大学, 医学(系)研究科(研究院), 准教授 (70250933) ; NISHIO Takeshi 京都大学, 医学(系)研究科(研究院), 助教 (70303790)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ケモカイン / 細胞膜小粒子 / 血小板 / 子宮内膜 / 再生 / 癌浸潤

Outline of Final Research Achievements

We designed this study based on the novel concept that platelet-derived factors induce endometrial epithelial cell migration and epithelial construction and its failure can cause onset and development of gynecological disorders. This study showed that the platelets promoted cell attachment of a human endometrial epithelial cell-derived immortalized cell line (EM-E6/E7/hTERT). Platelets inhibited, but the platelet-conditioned medium excluding the microparticle promoted EM-E6/E7/hTERT cell invasion. On the other hand, the platelet enhanced the human endometrial carcinoma-derived cell lines, Ishikawa cells and HEC-1 cells. These results indicate the possible involvement of platelets in endometrial epithelial regeneration and suggest differences in the responses to the re-epithelialization-inducing effects of platelet-derived microparticles between normal endometrial epithelial cells and cancer cells.

Free Research Field

生殖医学