Gene therapy for head and neck cancer using replication-selective adenoviral vector

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670717
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Kobe University
• Principal Investigator: KEN-ICHI Nibu 神戸大学, 医学(系)研究科(研究院), 教授 (20251283)
• Co-Investigator(Kenkyū-buntansha): OTSUKI Naoki 神戸大学, 大学院医学研究科, 准教授 (40343264)
• Co-Investigator(Renkei-kenkyūsha): SHIRAKAWA Toshirou 神戸大学, 大学院保健学研究科, 准教授 (70335446)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: gene therapy / head and neck cancer / adenoviral vector / replication-selective / siRNA / VEGF / Midkine

Outline of Final Research Achievements

A conditional replication-selective adenovirus vector in which the expression of E1a and E1b, required for viral replication, are controlled by the Midkine promoter, Ad-MK-E1, was generated.
To further enhance the oncolytic activity of Ad-MK-E1a, we integrated gene-expressing siRNA against VEGF to Ad-MK-E1 and generated Ad-MK-siVEGF. In vitro assays showed significant growth suppression not only in the cell lines expressing strong Midkine but also in the cell lines expressing weak Midkine, in comparison with adenoviral vector containing LacZ.

Free Research Field

耳鼻咽喉科頭頸部外科