2014 Fiscal Year Final Research Report
Cell therapy using iPS derived cells for hearing loss against the degenerations of various cochlear cell types
| Project/Area Number |
25670721
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Kazusaku 順天堂大学, 医学部, 准教授 (10374159)
IIZUKA Takashi 順天堂大学, 医学部, 助教 (40372932)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 聴覚障害 / 人工多能性幹細胞 / 内耳 / 有毛細胞 / ラセン神経節細胞 / 分化誘導 / 細胞治療 / 再生医療 |
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| Outline of Final Research Achievements |
In this study, we co-cultured iPS-derived cells with three different regions of the cochlear tissue and induced their differentiation into various types of inner cells. The number of Nanog-positive colonies with reporter GFP controlled by Nanog promotor was counted to assess the undifferentiated level of the cells. In the co-cultured cells with spiral ganglion, many cells with neurite-like protrusions were observed among the colonies. In the co-culture with the organ of Corti and spiral limbus (OC/SL), neuron-like cells were observed on the cell layer with tight junction-like adhesions. In the co-culture with the cochlear lateral wall, many cell layers with tight junction-like adhesions were observed, while undifferentiated Nanog-positive colonies were not observed. It was suggested that differentiation of iPS cells to cochlear cells was regulated depending on the part of the co-cultured organs.
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| Free Research Field |
耳鼻咽喉科学
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