2014 Fiscal Year Final Research Report
Retinal phosphoprotein analysis in novel Src mutant optic neuropathy mouse model
| Project/Area Number |
25670729
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
KATO Goro 山梨大学, 総合研究部, 助教 (60177441)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 網膜 / Ｓｒｃ / リン酸化タンパク質 |
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| Outline of Final Research Achievements |
Degradation of activated Src is regulated by Cdk5-dependent phosphorylation of Src Ser75. RGC loss was stimulated without elevated intraocular pressure in the aged phospho-mimicking mutant SrcSer75Asp mice (SD mice).To address the molecular mechanism of the degeneration induced by deregulation of the Ser75 phosphorylation, we established 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) to globally detect changes in the retinal phosphoprotein abundance in retinas. The level of spot #30, which had an apparent molecular weight of 45kDa and an isoelectric point of 5.5～6.5, in SD mice was increased significantly than that of wild type mice.These results can lead to identification of retinal phosphoproteins changed by the Src-specific phosphorylation mutation, and are very important in elucidating the mechanism of RGC degeneration.
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| Free Research Field |
分子細胞生物学
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