The development of a new way to regulate the cell state transition of cultured human corneal endothelial cells in regards to the possible RNA interference in infant corneas

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670736
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Toda Munetoyo 京都府立医科大学, 医学(系)研究科(研究院), 助教(特任講座) (30550727)
• Co-Investigator(Renkei-kenkyūsha): HAMURO JUNJI 京都府立医科大学, 医学系研究科, 教授 (80536095)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 角膜内皮細胞 / 再生医療 / miRNA / 細胞相転移

Outline of Final Research Achievements

We have defined the subpopulations (SPs) existing in the cultured human corneal endothelial cells (cHCECs) and identified the surface markers characteristic for each cHCEC SP, thereby have succeeded to establish the culture method to gain efficiently the high quality of cHCECs without cell state transition (CST). These SPs exhibited the distinctive proliferation propensity and features for energy metabolisms. The miRNA and the cytokines secreted to the culture supernatant (CS) were significantly different among these SPs. The CS of cHCECs without CST contained elevated levels of the selected species of miRNA, identical with those observed in CS of cHCECs of new born donors under one age old, indicating the relevance of miRNA interference present in infant HCECs.
All these findings altogether contribute to the elaboration to produce reproducibly the high quality of cHCECs without CST.

Free Research Field

医歯薬学