Transplantation of stromal vascular fraction in tendonregeneration

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670747
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: University of Tsukuba
• Principal Investigator: SEKIDO Mitsuru 筑波大学, 医学医療系, 教授 (40372255)
• Co-Investigator(Kenkyū-buntansha): SASAKI Kaoru 筑波大学, 医学医療系, 講師 (10536220) ; ADACHI Koji 筑波大学, 医学医療系, 講師 (70572569)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 腱組織再生 / マウスモデル / 間質血管細胞群移植

Outline of Final Research Achievements

Flexsor tendon injury of hand often results in limited hand functions.
In this study, we have developed a new simple murine Achilles tendon suture model with ankle fixation. Right Achilles tendons were incised and 10-0 nylons were passed through the proximal and distal ends with modified Kessler’s method. Then stromal vascular fraction in culture medium with Matrigel was applied in the SVF transplantation group, while only culture medium with Matrigel was applied in the control group. We recorded range of motion of plantarflexion of the ankles, and postoperative period until they started usage of the right Achilles tendons by their behavior. We revealed not only significant differences in those two factors, but also suppression of ectopic chondrogenesis which can lead poor quality of regenerating tendon.

Free Research Field

再建外科