Association of angiopoietin-1 genetic polymorphisms with altered clinical outcome in septic shock

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670758
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Chiba University
• Principal Investigator: NAKADA Taka-aki 千葉大学, 医学部附属病院, 講師 (20375794)
• Co-Investigator(Kenkyū-buntansha): HASHIDA Tomoaki 千葉大学, 大学院医学研究院, 特任助教 (60507375)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Septic shock / polymorphisms / vascular permeability

Outline of Final Research Achievements

Controlling endothelial permeability are important in pathogenesis of the complications and outcome of inflammatory processes such as septic shock. Polymorphisms of permeability genes could alter outcomes of septic shock. Thus, we hypothesized that polymorphisms of genes that modulate endothelial permeability are associated with increased percent fluid overload and mortality of septic shock. We genotyped SNPs of 16 permeability genes (ANGPT1, ROBO1, ROBO4, CTNNB1, SLIT2N, F2R, FLT1, TEK, S1PR1, S1PR3, KDR, NPPA, VEGFA, RAC1, CDH5, and PROCR) in 520 patients with septic shock. We tested for associations of SNPs with 28-day mortality and percent fluid overload. We evaluated organ dysfunction as secondary outcomes of explanatory interest. Only A allele of rs4324901 of angiopoietin-1 was associated with increased 28-day mortality and fluid overload. ANGPT1 rs4324901 AA genotype patients had increased respiratory and neurologic dysfunction and need for increased renal replacement therapy.

Free Research Field

救急集中治療医学