2015 Fiscal Year Final Research Report
Development of bacterial identification methods by volatile material analysis
| Project/Area Number |
25670768
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SEKINE Yoshika 東海大学, 理学部, 教授 (50328100)
ASAI Satomi 東海大学, 医学部, 准教授 (60365989)
MIYACHI Hayato 東海大学, 医学部, 教授 (20174196)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 広範囲重症熱傷 / 揮発性物質分析 / 環境揮発性物質 / 細菌同定 / アンモニア / アソシエーションルール / 多剤耐性アシネトバクター / 多剤耐性緑膿菌 |
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| Outline of Final Research Achievements |
In experimental model of volatile material analysis was done using 8 species bacteria such as MDRP, MDRA and others. Each bacterium has its own detection pattern of volatile materials. In results of volatile material analysis in 18 cases of severe burn patients, environmental ammonia concentration were correlated closely with clinical course of burn wound condition that ammonia concentration were increased in debridement and skin plantation, and decreased in wound closure. On the other hand, fatal cases were kept high. In analysis of wound gauze, volatile materials were able to separate human and bacterial source using by association rule. Volatile material analysis could distinguish genus of bacteria. However, large number of examinations must be done for decision of bacterial species.
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| Free Research Field |
救急医学
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