Communication between osteoblasts and osteoclasts in medaka

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670788
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Kudo Akira 東京工業大学, 生命理工学研究科, 教授 (70178002)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨折修復 / 骨モデリング / メダカ / 骨芽細胞 / 破骨細胞 / TGFb / 尾ひれ / 咽頭歯

Outline of Final Research Achievements

To investigate TGFb function during fracture healing in vivo, we used the fracture healing model of the medaka fish, which enabled us to observe the behavior and function of living cells in response to a bone-specific injury. TGFb-2 functions specifically during fracture healing to stimulate the migration of osteoblasts as well as the differentiation of osteoblasts and osteoclasts. Next, we developed the bone modeling system by employing the pharyngeal bones, coupled with the osterix-DsRed/TRAP-GFP transgenic line visualizing osteoblasts and osteoclasts. Our results demonstrated that bone formation and resorption sites are divided by the posterior and anterior sides of developed supporting bones, respectively, and osteoblasts lead to coordination of bone morphology through the support to osteoclasts.

Free Research Field

骨生物学