The individual difference in the dentin deterioration by Genetic Analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670804
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Hokkaido University
• Principal Investigator: Sano Hidehiko 北海道大学, 歯学研究科(研究院), 教授 (90205998)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Yasuhiro 北海道医療大学, 歯学部口腔機能修復・再建学系・う蝕制御治療学分野, 講師 (50431317)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: High Resolution Melt分析法 / ＤＮＡ抽出 / 個体差

Outline of Final Research Achievements

The dNA extraction procedure from pulp and the dentin specimens of tooth was able to get DNA of 9.8ng/μl and 5.8 ng/μl. In addition, High Resolution Melt analytical method (HRMA) analysis was performed for rs17576 SNP in MMP9. The frequency of the genetic polymorphism was also analyzed based on th Database of single nucleotide polymorphisms (SNPs) at the Naciotnal Center for Biotechnology Information. The frequency of A/A, A/G, G/G in the rs17576 SNP is 36.7% 44,6%, 18,7% . It was classified in three groups in the analysis by HRMA on the basis of data of NCBI equally by the data of 10004 samples. A method to analyze was established by dentin deterioration by genetic polymorphism by combining it with the load examination using the device for an automatic pH cycle.

Free Research Field

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