Effect of pressure change and simulated microgravity on the expression of pain receptors in cultured dental pulp cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670810
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: NAGATA Toshihiko 徳島大学, ヘルスバイオサイエンス研究部, 教授 (10127847)
• Co-Investigator(Kenkyū-buntansha): OISHI Keiji 徳島大学, 病院, 講師 (00253211)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 歯髄細胞 / 気圧変動 / 痛覚受容体 / マイクロアレイ分析

Outline of Final Research Achievements

This study investigated the effect of low-pressure atmosphere on the gene expression of pain-sensing mechanism of dental pulp cells. RPC-C2A cells derived from rat dental pulp were used to observe their changes under low-pressure atmosphere. The cells could grow under a 0.5 atmosphere pressure in a same growth rate as under a 1.0 atmosphere pressure. Microarray analyses showed that the cells had a same gene expression profile even under the low atmosphere environment for 8 days. Gene expressions of pain-sensing receptors were also unchanged under the environment. Similar pattern was observed even under 0.1 atmosphere pressure, whereas the change of other gene expression was observed. These results suggest that low atmosphere pressure is not directly affect on the pain-sensing mechanism of dental pulp cells.

Free Research Field

歯内治療学、歯周治療学