The novel strategy to regenerate periodontal ligament tissue using iPS cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670811
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Kyushu University
• Principal Investigator: AKAMINE Akifumi 九州大学, 歯学研究科(研究院), 教授 (00117053)
• Co-Investigator(Kenkyū-buntansha): WADA Naohisa 九州大学, 大学病院, 講師 (60380466) ; MAEDA Hidefumi 九州大学, 大学病院, 講師 (10284514) ; MONNOUCHI Satoshi 九州大学, 歯学研究院, 助教 (30609558)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 歯根膜組織 / iPS細胞

Outline of Final Research Achievements

In this study, we aimed to establish novel method to regenerate periodontal tissue by use of iPS cells. We examined the effects of new cell differentiation method and scaffold where cells attach and form tissue. When human periodontal ligament cells (HPDLCs) were cultured on the hybrid scaffolds, HPDLCs attached and proliferated well on the scaffold, and also formed extra cellular matrix tissue. In addition, co-culture of neural crest cells differentiated from iPS cells with HPDLCs in the presence of TGF-beta upregulated the expression of periodontal ligament-related markers, suggesting iPS cell differentiation into HPDLC-like cells. Although further studies are required, the combination of iPS cells induced to differentiate into neural crest-like cells and hybrid scaffold could be alternative cell source for novel cell transplantation method to regenerate periodontal ligament tissue.

Free Research Field

歯科保存学