2016 Fiscal Year Final Research Report
Boron accelerates cultured osteoblastic cell activity through calcium flux
Project/Area Number |
25670812
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Conservative dentistry
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Research Institution | Nagasaki University |
Principal Investigator |
HAYASHI Yoshihiko 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (20150477)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Keywords | Caイオンの移動 / 細胞増殖 / 細胞分化 / 骨芽細胞 |
Outline of Final Research Achievements |
The aim of this study was to investigate the effects of 0.1 mM of boron (B) on the membrane function of osteoblastic cells in vitro. The Ca2+ flux was evaluated for the activation of L-type Ca2+ channel for the Ca2+ influx, and that of Na+/K+-ATPase for the Ca2+ efflux. A real-time PCR analysis revealed that the expression of four mineralization-related genes was increased with a B-supplemented medium. Using microarray analyses, five genes involved in cell proliferation and differentiation were up-regulated. Regarding the Ca2+ influx, in the nifedipine-pretreated group, the relative fluorescence intensity for one min after adding B solution did not increase compared with that for one min before addition. Regarding the Ca2+ efflux, in 0.1 mM of B-supplemented medium, the relative fluorescence intensity for 10-min after ouabain treatment revealed a significantly lower slope value. B promotes the proliferation and differentiation of mammalian osteoblastic cells in vitro.
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Free Research Field |
齲蝕学、細胞生物学
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