Bone regeneration with bone substitute and cells in osteoclast lineage

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670834
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: IKEDA Tohru 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00211029)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA Masahiro 鹿児島大学, 医歯学総合研究科(歯学系), 教授 (00294570) ; KAMITAKAHARA Masanobu 東北大学, 環境科学研究科, 准教授 (80362854) ; IOKU Koji 慶応義塾大学, 経済学部, 教授 (60212726)
• Co-Investigator(Renkei-kenkyūsha): ERI Tatsukawa 長崎大学, 医歯薬学総合研究科(歯学系), 技術職員 (60534534)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨欠損 / 再生医療 / 破骨細胞 / 異物巨細胞 / 肉芽腫

Outline of Final Research Achievements

We tried to show importance of osteoclasts for bone regeneration in the region implanted with bone substitutes and osteoblast progenitor cells. We implanted granules of biodegradable bone substitute, beta tricalcium phosphate (β-TCP) in rat subcutaneous tissue with or without bone marrow mesenchymal cells, which include osteoblast progenitor cells. Implantation of bone marrow mesenchymal cells induced not only osteogenesis, but also osteoclastogenesis. Osteoclastogenesis stimulated resorption of β-TCP. These results showed that implantation of bone marrow mesenchymal cells stimulated replacement of bone substitute to the bone. In addition, we found that hydroxyapatite composed of rod shaped particles induced osteoclast-like multinucleated giant cells without implantation of bone marrow mesenchymal cells. Furthermore, we showed divergence of multinucleated giant cells induced around hydroxyapatite granules implanted in the rat subcutaneous tissue.

Free Research Field

口腔病理学